MBIO 2360 Study Guide - Quiz Guide: Glycolysis, Galactose, Escherichia Coli

• School: University of Manitoba
• Department: Microbiology
• Course: MBIO 2360
• Professor: Sean A.Mc Kenna

Document Summary

0. 01 l i n m e o m u e t a. Michaelis-menten graph showing initial rate, vo, of e. coli -galactosidase and a. oryzae -galactosidase versus the concentration of onpg in a solution of -gal buffer, varying concentrations of onpg and 50 l of e. coli -galactosidase/a. oryzae -galactosidase . Onpg consisted of 6. 0 mm 2-nitrophenyl- d galactopyra(cid:374)oside i(cid:374) gal buffer. l o m u n m i. 0 y = 36. 315x + 51. 789 y = 3. 9667x + 64. 777. Lineweaver-burk graph showing the relationship between the inverse of the initial rate of e. coli -galactosidase and a. oryzae -galactosidase and the inverse of the varying concentration of onpg. Comparing values of vmax and km for e(cid:374)zy(cid:373)e -galactosidases from e. coli and a. oryzae for onpg. 0. 697 l o m u n m i. The lineweaver-burke plot for the inverse of the initial rate of e. coli - Galactosidase and a. oryzae -galactosidase and the inverse of the varying concentration of.