MBIO 3030 Study Guide - Final Guide: Biosafety Cabinet, Biosafety, Fume Hood

• School: University of Manitoba
• Department: Microbiology
• Course: MBIO 3030
• Professor: Richard Sparling

Document Summary

This technique is used for novice microbiologists, it works well for isolating colonies but is very time consuming and impractical when using disposable pipettes. New method: fla(cid:373)e the loop, dip it i(cid:374)to (cid:272)ultu(cid:396)e a(cid:374)d tou(cid:272)h the side of the test tu(cid:271)e (cid:449)all (cid:894)do(cid:374)"t want a whole loopfull, as it will be too much. Part b serial dilutions and spread plate. Dilute till -8 using a new pipette every time. Plate the last 3 dilution onto t-soy plates (0. 1 ml). Can use the same pipette if you start from the most diluted sample and work up. Use alcohol sterilized spreader for spread plate technique. Before air is exhausted from the cabinet it passes through a hepa filter that functions to remove any airborne pathogens: to provide an aseptic workspace. Because air flowing through work area has already passed through a hepa filter, it should be free of airborne microbes.