ACB 406.3 Study Guide - Fall 2018, Comprehensive Midterm Notes - Connective Tissue, Collagen, Basal Lamina

• School: U of S
• Department: Anatomy and Cell Biology
• Course: ACB 406.3
• Professor: Helen Nichol

Document Summary

Commonality in developing cells: plasma membrane, cytoplasm, membrane-bound organelles, non membrane bound: cytoskeletal elements, centrioles, ribosomes, nucleolus, lipid droplets. Cells usually change morphology as they develop and differentiate: Overall size: neurons increase in size, rbcs decrease in size. # of nuclei: skeletal muscle cells acquire nuclei, rbcs lose nuclei. Amount and type of extracellular matrix: e. g. bone and cartilage. Polarity = discrete apical and basal surfaces. Stains: hematoxylin and eosin (h and e) Ve charged dna, rna backbone, proteins in chromatin, calcified matrices, rer. Eosin = binds to eosinophilic structures: +ve charge protein side chains. Periodic acid schiff pink = basement membrane, glycogen. Copper phthalocyanine = lipoproteins of the myelin sheath. Microscopes: light microscopy, phase contrast = living preparations; organelle movements, fluorescence = gfp; intravital dye; fluorescent antibodies, nomarski differential interference. Nucleus = they (cid:272)o(cid:374)for(cid:373) to the (cid:272)ell"s shape: heterochromatin = co(cid:374)de(cid:374)sed (cid:862)i(cid:374)a(cid:272)ti(cid:448)e(cid:863, eu(cid:272)hro(cid:373)ati(cid:374) = u(cid:374)(cid:272)oiled (cid:862)a(cid:272)ti(cid:448)e(cid:863) Sc are rich in rough er are stained purple er w/ ribosomes.