BIOL130L Study Guide - Final Guide: Relative Permittivity, Amylopectin, Centrifugation

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6 Feb 2016
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BIOLOGY 130L- Lab Summaries
Lab 1: Identification of Some Macromolecules
There are four types of macromolecules: carbohydrates, lipids, proteins and nucleic acids.
Expected Results:
Beaker #: Iodine Benedict Biuret
1 - + (red-brown) -
2 - - -
3 - + (green-brown) -
4 - +(orange-brown) -
5 - - -
6 - +(red-brown) -
7 + (reddish) - -
8 + (blue) - -
9 - - +
10 - + (green-brown) -
11 - - -
12 UNKNOWN UNKNOWN UNKNOWN
Honey and Beer (4 and 10) can give FALSE protein results because of their original colour.
Iodine Test: Tests for starch, amylose, amylopectin and glycogen. Starch and amylose gives a
blue/black colour. The others give a brown/purple colour.
Benedict’s Test: Tests for reducing sugars (maltose, lactose, glucose). Produces precipitate
ranging from red, yellow, green, orange and brown.
Biuret’s Test: Tests for proteins- turns purple upon detection.
Lab 2: Isolation of Some Macromolecules
· Learn every step of this lab and why it is done. (ie what is being isolated, centrifuged, dissolved,
etc.?)
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· Centrifugation: separation by density resulting in supernatant (liquid at the top) and pellet (solide
at the bottom).
· Purpose of adding salt is to neutralize negative charge on DNA by forming a positive cloud
around it (salt dissociates into + and – ions)
· Ethanol is used for precipitation as it has a lower dielectric constant than other polar organic
solvents. This increases the precipitation.
Lab 3: Characterization of Some Macromolecules
-Proteins:
· success= only amino acids in “hydrolyzed proteins” fraction; intact proteins in ‘unhydrolyzed’
· unsuccessful= mixture of proteins, peptides and amino acids
· unknown amino acid is alanine
Nucleic Acids:
· success= bases
· unsuccessful= intact RNA
Lab 4: Spectroscopy
Dilutions:
1) 0.015 mg/mL
2) 0.0075 mg/mL
3) 0.00375 mg/mL
4) 0.001875 mg/mL
5) 0.0009375 mg/mL
Maximum Absorbance for Fast Green= 635 nm
Chloroplast Separation (from top to bottom):
Orange band= carotene
Yellow band- xanthophyll
Yellow band- xanthophyll
Green (green) band- chlorophyll a
Green (blue) band- chlorophyll b
Lab 5: Enzymes
Results of the Iodine and Benedict’s Tests
Test Tube # Contents Iodine Test Benedicts Test
1 10% Amylase - -
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