Study Guides (256,147)
CA (124,565)
UW (5,910)
BIOL (1,124)
BIOL239 (69)

BIOL 239 Quiz: Set 20- Genetic Analysis

7 Pages

Course Code
Christine Dupont

This preview shows pages 1 and half of page 2. Sign up to view the full 7 pages of the document.

Loved by over 2.2 million students

Over 90% improved by at least one letter grade.

Leah — University of Toronto

OneClass has been such a huge help in my studies at UofT especially since I am a transfer student. OneClass is the study buddy I never had before and definitely gives me the extra push to get from a B to an A!

Leah — University of Toronto
Saarim — University of Michigan

Balancing social life With academics can be difficult, that is why I'm so glad that OneClass is out there where I can find the top notes for all of my classes. Now I can be the all-star student I want to be.

Saarim — University of Michigan
Jenna — University of Wisconsin

As a college student living on a college budget, I love how easy it is to earn gift cards just by submitting my notes.

Jenna — University of Wisconsin
Anne — University of California

OneClass has allowed me to catch up with my most difficult course! #lifesaver

Anne — University of California
GeneticAnalysis  Things to recall with a library? o Libraries are used to track a specific fragment, but in order to determine which cell has it use probes to bind to complimentary gene areas (probes are single stranded) o They isolate fragments that wouldn’t normally be isolated as they are in a smear)  Describe probes in more detail o Short single-stranded stretches of DNA of known composition, form 25 to several thousand nucleotides in length o 1) To visualize them, the probes are labeled, usually with 32P or fluorescent dyes Used in the identification of clones that contain complementary DNA sequences. From 25 to several thousand nucleotides in length o Several ways to make labeled probes Can be chemically synthesized, and are termed oligonucleotides (usually 25 to 100 nucleotides in length). Fluorescent dyes can be added as part of the synthesis procedure. o Must know the DNA sequence of the probe o 2) Can label a previously cloned fragment  Removal from the vector by restriction endonuclease digestion, followed by chemical labeling  usually 100 - 500 base pairs in length  Don't need to know the sequence of this probe  Error! Filename not specified.  Describe screening through hybridization o Isolating gene Replica plating allows to find exact l ocation on master plate o Nylon adheres to DNA, all junk can be washed away o NaOH used to break hydrogen bonds and make DNA single stranded o This break allows probe to bind to complementary gene o Look where different on replica plate, see where is on master plate to find gene of interest  Get mRNA for gene of interest --> Make it cDNA (single stranded) --> Get colony library which has normal DNA and your inserted vector from before --> Replica to nylon --> Kill cells to expose DNA --> NaOH to make single --> add cDNA probes --> Wash away non annealled stuff --> X ray to see where colony with vector of interest is located --> Refer to master plate  Error! Filename not specified.  Evaluate screening through hybridization o The region of complementarity between probe and target sequence must be long enough to allow sufficient hydrogen bonds to provide a cohesive force o In general, two single DNA strands that are longer than 50-100 bp will hybridize so long as the extent of their complementarity is more than 80% needs to fit both parameters to work  Error! Filename not specified.  Describe Southern Blot Analysis o Used to show the presence of a gene within a larger fragment of DNA Involves the transfer of DNA fragments from an agarose gel to nitrocellulose filter paper o The relative position of the DNA fragments is preserved in the transfer Hybridization between a specific probe and DNA on the filter can be carried out o Not isolating gene, just want to see if have it o Nylon membrane makes stick in place (not diffuse once electrogradient is removed) render single stranded w/ NaOH o Capillary action of buffer below goes up imprinting DNA to nylon membrane. Paper towels for capillary action o Shows if an individual has a gene, if so how frequent is it?  Error! Filename not specified.  Describe PCR o Artificial DNA xeroxing o With the use of 2 specific oligonucleotides (primers), any fragment of DNA can be amplified from 1 to ~ 1 billion copies in as little as 2 hr. o PCR primers need to be 100% correct, so need to know sequence o Has uses in DNA cloning, DNA sequencing, making DNA probes, identification of organisms w/o Southern blotting o Amplifies up gene region in between two primers. Design two primers that are complementary to each end of the sequence of interest. o Exponentially more DNA strands are made o Primers have their 3' facing target region o Mix the primers, template DNA, thermostable DNA polymerase (e.g. Taq) and thenucleotide bases in a microtube Begin thermocycling procedure  Error! Filename not specified.  Describe PCR cycles  Error! Filename not specified.  Why is PCR exponential?  Each cycle of PCR results in duplication of the strands, exponential  Error! Filename not specified.  Uses for PCR o Diagnosis of infection to see a gene exclusive to a specific bacteria o Paleomolecular biology to see what infectious disease they had back then  Describe DNA sequencing, Sanger sequencing? o Provides the highest resolution of a cloned DNA fragment: A complete description of its genetic information at the level of the nucleotide o Requires a
More Less
Unlock Document

Only pages 1 and half of page 2 are available for preview. Some parts have been intentionally blurred.

Unlock Document
You're Reading a Preview

Unlock to view full version

Unlock Document

Log In


Don't have an account?

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.