Study Guides (248,368)
Canada (121,499)
Biology (1,112)
BIOL 240 (75)

Complete Notes for BIOL 140L Exam, Lab Quizzes

7 Pages
Unlock Document

BIOL 240
Cheryl Duxbury

Bio 140L NotesAaron Tai1 Experiment 1Stains a Dyes have cationic chromophore colour i Loefflers Methylene bluemethylene and chloride choromophore b Bacteria have negative charge on outside of their membrane which attract to positive stain chromophores like above c Used to determine morphology of bacteria 2 Microscope a 3 partslens system objective ocular light source geared mechanism adjust distance from lens system and object observedcourse fine adjustment knobs i Objectiveproduce magnify specimen to make image project to ocular 1 4x scanning 10x low 40x high 100x oil immersion ii Ocularfurther magnify produce virtual image for eyes 1 10x b Oilimprove resolution of image c Iris Diaphragmcontrol amount of light entering condenser d Mechanical stageholds slide e Condenserlenses that collectconcentrate light reaching stage 3 Bacterial Shapes a Rodbacillusmotilesingly pairs diplobacillus chains streptobacillusb Roundcoccusnonmotilesingly micrococcus pairs diplo chains strepto 4group gaffkya 8group cube sarcina clusterpacket staphylo c Spiralspirochetespirilliummotile d StalkedCaulobacter e Clubshapedcorynebacterium f Vibriocommashaped g Page 20 chart h Magnificationsize of specimen in drawingest actual size of image 4 Gram Staindifferential stain a Mordantsubstance that fix primary stain to cells eg iodine b Decolourizing agentethyl alcohol c Counterstainsafranin d Gram purple violet crystal e Gram pink safranin f Results can be affected by pH and age of culture mediumgram variable organism g Procedureprimary stain crystal violet mordant iodine to fix primary stain decolourize with alcohol counterstain safranin1
More Less

Related notes for BIOL 240

Log In


Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.