BIOL240 Study Guide - Quiz Guide: Lactobacillus Plantarum, Serial Dilution, Potassium Alum

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coffeegnat887

3 Feb 2016

School

University of Waterloo

Department

Biology

Course

BIOL240

Professor

Cheryl Duxbury

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Document Summary

Experiment 11: streak-plate method isolation of pure culture. Pure culture: culture consisting of only type of microorganism. 3 dilution methods commonly used for the isolation of bacteria: streak plate essential dilution technique that spreads a loopful of culture over the surface of an agar plate, spread plate, pour plate. Dilute the specimen into a series of cooled (45-50 degree) fluid-agar medium which is then poured into empty petri plates. It is necessary to make several dilutions to ensure that at least one countable plate will be obtained on which distinct and separate colonies have formed on, or in, the agar medium. Used mixed cuture of escherichia coli and staphylococcus aureus. Practice in handling pipettes in an aseptic manner. Sterile technique will be measured by the absence or presence of bacterial growth in nutrient broth. Pipettes are frequently used to transfer bacterial suspensions or to create serial dilutions.

Related Questions

Considering the cultures to be used for inoculating each medium in this exercise, how many microbial types should you expect to see in/on each medium?

Select one:

a. 3

b. 0

c. 1

d. Several

e. 2

Which of the following is common to minimize airborne contamination?

Select one:

a. Make someone else do the experiment

b. Perform the experiment in a windy room

c. Keep a microscope nearby

d. Breathing in sample

e. Hold open tubes at an angle

How can a hot sterile flame loop be cooled quickly to use in the next streak?

Select one:

a. Put in sterile portion of agar plate

b. Blow on it

c. Stick the loop in ice

d. Set the loop on the lab bench

e. Pour tap water on the loop

True or false: Most colonies can been seen without aid (naked eye), and most single cells cannot be seen without a microscope.

Select one:

True

False

An isolated, single colony forms after incubation of a:

Select one:

a. Virus (viral particle arrangement in some cases)

b. Single cell (cell arrangement in some cases)

c. Sterile agar plate

d. Sterile flame loop

e. Liquid mixed culture

These are commonly used for growing stock cultures that can be refrigerated for several weeks:

Select one:

a. Broth tubes

b. Cotton swabs

c. Gym socks

d. Agar slants

e. Petri dishes

Beginning at the base of the slant surface and gently moving the loop back and forth as you withdraw is called:

Select one:

a. Slant mixing

b. Fishtail inoculation

c. Zig-zag streaking

d. Squirrel inoculation

e. Quadrant streaking

If you are going to mix a broth culture prior to transfer, what is a safe way to do this without special equipment?

Select one:

a. Tap the tube with your fingers

b. Vortex it

c. Use a inoculating loop

d. Stick the tube in the outer cone

What is the primary purpose of the streak plate method?

Select one:

a. Quantify CFUs

b. Isolate an individual colony

c. Quantify the cell count of a mixed culture

d. Inoculate a culture on a plate

e. Confirm the identity of an unknown genus

BIOL240 Study Guide - Quiz Guide: Lactobacillus Plantarum, Serial Dilution, Potassium Alum

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