BIOL240L Study Guide - Final Guide: Gram Staining, Aspergillus Niger, Vibrio

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Chromophore is found on positive staining procedures and they are basic. Produces a pronounced attraction between the chromophores and organism. Heat fixing used to make sure the cells stay on the cell. Find the magnification by multiplying ocular by objective. Emersion used to improve resolution of the image, done by high refraction index, increasing numerical aperture. If dim or too bright, adjust power/intensity control, check to make sure that iris diaphrahm is half open, check condenser is at top of travel. Clusters for coccus is staphylococcus, chains in bacillus are called streptobacillus. Gram stains differentiate between types of bacteria depending on their abilities to retain a particular stain. Mordant fixes the primary stain in the bacterial cells (iodine), forming a complex between the crystal violet and cell nucleic acid. Only fresh culture should be used because age and ph can affect reaction gram stain. Staphylococcus epidermis is gram-positive and formed in groups.