BIOL266 Study Guide - Midterm Guide: Protein Sequencing, Sanger Sequencing, Central Dogma Of Molecular Biology

• School: University of Waterloo
• Department: Biology
• Course: BIOL266
• Professor: Andrew C Doxey

Document Summary

Objective: to explore basic database theory and special implications for biological data. History of sequencing: protein sequencing, 1955: first complete protein sequence (insulin, ryle et al. Genbank; it is still the predominant source of genomic info on the web. Genome sequencing: with advancement of molecular and bioinformatic techniques came the desire to sequence entire genomes, genomes sequenced, ba(cid:272)teriophage x1(cid:1011)(cid:1008) (cid:894)(cid:1009)(cid:1007)(cid:1012)(cid:1010) (cid:271)p(cid:895) sanger et al. 1(cid:1013)(cid:1011)(cid:1011) very first sequenced genome: human mitochondria (~16 kb) anderson et al. 1981: nicotiana tabacum & marchantia polymorpha chloroplasts (120-200 kb, first eukaryotic chromosome: chromosome iii of saccharomyces cerevisiae, first bacterial genome was sequenced in 1995 (haemophilus influenzae) at tigr (1. 8. The sequence revolution: development of these te(cid:272)h(cid:374)i(cid:395)ues laid fou(cid:374)datio(cid:374) fo(cid:396) the (cid:862)se(cid:395)ue(cid:374)(cid:272)e (cid:396)e(cid:448)olutio(cid:374)(cid:863) of the (cid:1005)(cid:1013)(cid:1012)(cid:1004)s and 1990s, development of more efficient computer technology. Hardware and software: the combination of the above resulted in the birth of the field of bioinformatics. Ddbj: japanese database; was established slightly earlier than genbank.