BIOL308 Study Guide - Final Guide: Constitutive Heterochromatin, Crystallin, Recbcd

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Isolate the genomic clone, sequence it and determine the aa sequence. Temperature sensitive mutants exhibit a wild type phenotype under low temps and exhibit a mutant phenotype at higher temps. Thus, conditions are permissive when the mutants are allowed to grow normally or at low temps. When a temperature sensitive mutant is grown in a permissive condition, the mutated gene product behaves normally (meaning that the phenotype isn"t observed), even if there is a mutant allele present. This results in the survival of the cell or organism, as if it were a wild type strain: define (or compare and contrast): a) gene expression; transcription; replication; translation, gene; allele. Gene expression is the process by which the information from a gene is used in the synthesis of a functional protein or rna product. Processes through which the information carried by a gene is converted into observable product.

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