BIOL308 Study Guide - Final Guide: Glutamine, Rate-Determining Step, Homeobox
23 Apr 2018
School
Department
Course
Professor
Document Summary
Pro transcription and translation coupled euk occurs in dif compartment than translation. That there are multiple rnaps involved in transcription in eukaryotes and that each. Enhancers, elements that stimulate transcription usually from a long distance away. Identified by activators bound to it which stimulate initatie. Can be found anywhere, different from upstream which is usually in close proximity to initation sequence and is also upstream: explain the use of reporter genes for estimation of promoter strength. Reporter genes are a coding unit that has product that is easily viewable and differentiated from other proteins in the cell. Promoter from a gene of interest is insert upstream of a gene with easily observable product, inserted into the cell, product measured in order to determine strength of the promotor. Product produced proportional to strength of the promoter: explain briefly 5" deletion series.
