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BIOL308 Study Guide - Final Guide: Nucleobase, Covalent Bond, Phosphodiester Bond

4 pages137 viewsSpring 2018

Course Code
Dragana Miskovic
Study Guide

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Study questions for the lectures 1-4
1. What information(s) could you obtain from a genetic approach of studying mutants
defective in a particular process?
Potential function of the protein and if it is necessary for survival
The number of genes thus proteins involved in a certain process
The order of genes and their relation to each other
How genes interact with each other
2. How would you define permissive conditions in respect to temperature sensitive mutants?
3. Define (or compare and contrast): a) gene expression; transcription; replication; translation;
b) gene; allele
Gene expression a process through which information being carried by the gene is
converted into observable product
Transcription a process through DNA is copied to form a single stranded RNA which is
identical to one of the strands of the duplex DNA
Translation process through which the RNA nucleotide sequence is converted into an
amino acid sequence of a protein
Replication process of making an exact copy of DNA from an original DNA molecule
1. Gene functional unit of heredity, the dna sequence necessary for synthesizes an RNA
molecule or functional polypeptide
2. Allele, alternative forms of a single gene
4. Explain by using your own words the meaning/significance of gene expression.
Gene expression is significant because the end product of gene expression is protein synthesis,
everything is made of proteins
5. What are the roles of model organisms in molecular biology studies? Choose two model
organisms and explain your reasoning.
The role of model organisms is to use biochemical and genetic approaches of studying
gene expression on them in order to understand how gene expression functions, model
organisms are used because they are cheap, small, easy to use, have short reproduction
cycles, results can be extrapolated into larger more complex organisms
One model organism is C. Elegans which are used to study affect of gene expression on
development, have small number of cells, every cell can be counted, good model
Another model organism is e coli which has simple nutritional needs, good model
organism, divide quickly, have many different proteins and base pairs
6. What are three main functions of DNA? Explain the importance of each of them.
Store information genetic information is carried by dna sequence which is necessary for
making a functional protein, the information stored by the sequence goes on to form a
functional protein, everything is about proteins in the end
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