BIOL308 Study Guide - Final Guide: Nucleobase, Covalent Bond, Phosphodiester Bond
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23 Apr 2018
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Ionic ineractions between positively charged salts and the negative backbone of the. A dna, much wider and shorter, a slight tilt, found in environment with low water conc, lower than normal in cell, can occur in dormant spores. If you want to denature dna, you"d have to be able to pull the two strands apart from eachother. Tm of dna will be much higher in higher salt ceoncentration, meaning a much higher melting temp, point at which half of dna is denatured, in order to overcome to neaturalized charges. This is is due to the fact that double stranded dna is organized and bases are stacked (hypochromic effect) meaning that light passes through it easily and not much is absorbed. In ssdna, the bases go in all directions and they are not stacked (hyperchromic effect) meaning they catch light and absorb much more of it.
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