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BIOL 308 Final: Study_questions_1

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Dragana Miskovic

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Study questions for the lectures 14 1. What information(s) could you obtain from a genetic approach of studying mutants defective in a particular process? Potential function of the protein and if it is necessary for survival The number of genes thus proteins involved in a certain process The order of genes and their relation to each other How genes interact with each other 2. How would you define permissive conditions in respect to temperature sensitive mutants? 3. Define (or compare and contrast): a) gene expression; transcription; replication; translation; b) gene; allele Gene expression a process through which information being carried by the gene is converted into observable product Transcription a process through DNA is copied to form a single stranded RNA which is identical to one of the strands of the duplex DNA Translation process through which the RNA nucleotide sequence is converted into an amino acid sequence of a protein Replication process of making an exact copy of DNA from an original DNA molecule 1. Gene functional unit of heredity, the dna sequence necessary for synthesizes an RNA molecule or functional polypeptide 2. Allele, alternative forms of a single gene 4. Explain by using your own words the meaningsignificance of gene expression. Gene expression is significant because the end product of gene expression is protein synthesis, everything is made of proteins 5. What are the roles of model organisms in molecular biology studies? Choose two model organisms and explain your reasoning. The role of model organisms is to use biochemical and genetic approaches of studying gene expression on them in order to understand how gene expression functions, model organisms are used because they are cheap, small, easy to use, have short reproduction cycles, results can be extrapolated into larger more complex organisms One model organism is C. Elegans which are used to study affect of gene expression on development, have small number of cells, every cell can be counted, good model organism Another model organism is e coli which has simple nutritional needs, good model organism, divide quickly, have many different proteins and base pairs 6. What are three main functions of DNA? Explain the importance of each of them. Store information genetic information is carried by dna sequence which is necessary for making a functional protein, the information stored by the sequence goes on to form a functional protein, everything is about proteins in the end
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