BIOL308 Study Guide - Polyadenylation, Cistron, Insertion Sequence

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Protein product of l1 element may create dna dsbs. Alternatively, existing dsb may be repaired by non-classical insertion of a retrotransposon. Gene conversion may alter sequence compositions of homologous retrotransposon copies. Insertion associated with deletion of target genomic sequence. Ectopic recombination between non-allelic homologous retrotransposons may result in genomic rearrangements e. g. , deletions or duplications: list and briefly describe three mechanisms used by genetic elements to randomly move from one site in the genome to another. Dna-only transposons - example: insertion sequence (is) elements: encode only genes for mobilization and insertion (host replication machinery used for replication). Retrotransposons (retroviral-like) carry long terminal repeat sequences (ltrs) and two genes important for recombination (rt and integrase). Cannot move from cell to cell; move only to new dna within the cell. Nonretroviral retrotransposons or polya retrotransposons: - poly a at 3" end of rna transcript; Moves via rna intermediate that is often produced from a neighboring promoter.

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