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BIOL 308
Bruce Wolff

1You have discovered base changes in the promoter region of the operon in a bacterial chromosome Would you expect these changes to act in trans on another copy of the operon Explain your reasoning2What are cis elements What are trans factors Give an example from the Trp operon or form the Araoperon3Draw the diagram of the lac operon that illustratesbe carefulnegative control here and think about the complete picture4You have isolated a protein that binds to DNA in the region upstream of the promoter sequence of the gene of interest If this is a positive regulator activator which would be trueA Loss of function mutation in the gene encoding this DNA binding protein would cause constitutive expressionB Loss of function mutation in the gene encoding this DNA binding pro
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