BIOL308 Study Guide - Final Guide: Kozak Consensus Sequence, Transcription Preinitiation Complex, N-Formylmethionine
11 Jan 2015
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Document Summary
It is the site for prokaryotic ribosomal binding, as recognized by the 3" of 16s rrna of the 30 s subunit. It is the proper sequence for the eukaryotic 43s ribosome with initiator trna to scan to, thus properly positioning the ribosome at the start site. Prokaryotes have formyl-methionine initiator trna, while eukaryotes have just methionine. 43s initiation complex reaches the kozak aug, after which gtp is used, factors are released, and the 40s initiation complex forms. The gtpase complex does not bind as a ternary complex, as in eukaryotes, although gtp is used for the initiation complex to be formed. binding of trnai initiates factor release, allowing 50s to bind. In eukaryotes, the trna binds before the preinitiation complex binds the mrna. In prokaryotes, the preinitiation complex binds mrna, and then initiator trna enters the p site. Describe in detail events during initiation of the eukaryotic (or prokaryotic) translation.
