Gene expression: information carried by a gene converted into an observable product. Gene: entire dna sequence needed for the synthesis of a rna molecule or functional polypeptide including coding and regulatory regions. Detect for particular gene of interest in genome + how many copies and the location. Rna/protein is used to determine if the gene is turned on , when it is expressed and in what tissues, change of expression in correlation with disease. Expression target gene is assessed by observing abundance of rna transcribed and amount of protein translated. Nucleic acid hybridization: detecting for complementary dna or rna: southern blotting: dna detection, northern blotting: rna detection, western blotting: protein detection. Four general concepts for detection of nucleic acids and proteins: need a hybridization probe detect specific gene/product: nucleotides complementary to the gene/mrna of interest (dna; southern) or mrna (northern). Homologous probe: 100% complementarity to probe, occurs when probe is made from a.
