BIOL483 Study Guide - Midterm Guide: Spectrophotometry, Microcell, Diethylethanolamine
7 Sep 2013
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Document Summary
Cell cultures are to be monitored to assess the condition of the culture. Cells can be counted manually or electronically. Consists of thick glass slide with a grid. Cells are counted and the total number of cells/ml can be determined. Solution is drawn up through a tube, creating a pulse that is detected by the counter. We can set an upper and lower size limit of particles to avoid particles that aren"t the size of the cells that you want to count. Protein determination: measures the total amount of protein, protein levels in cells can vary, bradford assay is used to determine the protein content. Glucose determination: measures the changes in glucose levels with respect to cell density, this technique is a good choice for immobilized cells, when it is hard to isolate cell samples in bioreactors, due to immobilization. Cell viability measures the metabolic state of a cell population.
