BIOL 483 – Study Questions
1. What is animal cell culture?
The maintenance and growth of cells from animals in a man-made environment
2. Who developed cell culture and what did he do?
Ross Harrison in 1907
Cultured frog nerve cells using the ‘hanging drop’ technique and a lymph clot
3. What were the major findings of this research?
There were three major findings:
o The cells in culture required a support for growth anchor for cell
o The cells in culture required nutrients these nutrients were derived from the
o The cells in culture grew slower than bacteria there is a greater likelihood
of contaminating the culture with bacteria due to slower growth of the cells
4. What is the basic equipment you need to culture animal cells?
There are four main requirements:
o Sterile environment to manipulate cultures
o Proper culture environment to support cell growth
o Nutrient environment to support cell growth
o Appropriate physical support
Minimum basic equipment:
o Microscope (inverted)
5. The n