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Study Guides for BIOL484 at University of Waterloo

Advanced Eukaryotic Genetics

BIOL484 Study Guide - Midterm Guide: Salivary Gland, Microrna, Noncoding Dna

and G phases, has been called the endocycle. The endocycle occurs in almost all larval tissues, but is extensive in the larval salivary gland of many Diptera (flies) Some limitations of chisquare First, chisquare is highly...

Biology
BIOL484
Bruce Reed
BIOL484 Study Guide - Midterm Guide: Polytene Chromosome, Endoreduplication, Trophoblast

There was already an indication that genes paralleled chromosomes. If we could somehow change or manipulate the manner in which chromosomes behaved, then we should see a corresponding and change in inheritance patterns (ge...

Biology
BIOL484
Bruce Reed
BIOL484 Study Guide - Midterm Guide: Spindle Apparatus, Centromere, Synapsis

Tetrad- a group of four chromatids formed by synapsis at the beginning of meiosis. Dyad- One pair of homologous chromosomes resulting from the division of a tetrad during meiosis. Sister chromatid...

Biology
BIOL484
Bruce Reed
BIOL484 Study Guide - Midterm Guide: F1 Hybrid, Mendelian Inheritance, Nondisjunction

Mendels big ideas Mendel realized that sexual reproduction involves two parents and suggested that there are two inheritable factors for any given trait. (This is part of Mendels second BIG idea.) Inheritable fact...

Biology
BIOL484
Bruce Reed
BIOL484 Study Guide - Midterm Guide: Agarose Gel Electrophoresis, Restriction Digest, Chromosome

The first step in mapping a mutant gene is to assign the gene to a broad region of one chromosome. The method presented here uses 48 SNPs evenly spaced along the chromosomes, allowing the whole genome to be scanned simult...

Biology
BIOL484
Bruce Reed
BIOL484 Study Guide - Midterm Guide: Outcrossing, Nondisjunction, Restriction Enzyme

1) Meiosis is different worms create localized microtubule attachment sites 2) In many organisms, one crossover decreases the likelihood of another crossover nearby, an enigmatic process called crossover interference. In C...

Biology
BIOL484
Bruce Reed
BIOL484 Study Guide - Midterm Guide: Gene Gun, Microparticle, Microinjection

A significant advance in nematode transgenics was the discovery that microparticle bombardment can induce integrative transformation in C. elegans. With this technique, DNA is bound onto gold particles which are then "shot...

Biology
BIOL484
Bruce Reed
BIOL484 Study Guide - Midterm Guide: Transgene, Syncytium, Intron

Using p element can be mobilized with delta 23. has coding sequence flanked by internal exon boundaries. Selected to be strong splicing sites. If p element happens to intergrate into an intron- get rna produced gets splice...

Biology
BIOL484
Bruce Reed
BIOL484 Study Guide - Midterm Guide: Flippase, Fusion Protein, Transposable Element

The FLPFRT system can generate twin spots If two FRT sites are inserted via a transposon at two identical sites (between non homologous chromosomes ) then when FLP flippase is induced by the heat shock promoter it is under...

Biology
BIOL484
Bruce Reed
BIOL484 Study Guide - Midterm Guide: P Element, Progenitor Cell

The ep ELEMENT The EP element is a variation of the UAS /GAL4 system. Here the random insertion of a P element loaded with UAS sequences can render a neighbouring gene sensitive to GAL4 control. Note that the EP element m...

Biology
BIOL484
Bruce Reed
BIOL484 Study Guide - Midterm Guide: Transgene

Gal4 UAS system In one construct, a cell-specific promoter drives the expression of the gene encoding Gal4, a transcription factor normally expressed in yeast. In a second construct, a transgene of interest is regulated ...

Biology
BIOL484
Bruce Reed
BIOL484 Study Guide - Midterm Guide: Beta-Galactosidase, Immunohistochemistry, Chromogenic

turned on due to the fusion with the promoter. This will ultimately result in a blue colour being produced when xgal the substrate of betagalactosidase is added to the media. This is called enhancer trapping. Im assuming h...

Biology
BIOL484
Bruce Reed
BIOL484 Study Guide - Midterm Guide: Beta-Galactosidase, Transposable Element

This method uses reporter genes that can be inserted randomly within the g enome. When a reporter gene is placed near the enhancer, the reporter gene product is detected within the cells. Steps involved in enhancer trap te...

Biology
BIOL484
Bruce Reed
BIOL484 Study Guide - Midterm Guide: Tata Box

to balance Xlinked lethal mutations, X chromosome balancers must be viable and fertile in males but carry a recessive female sterile mutation good notes: A challenge of Drosophila is they cannot be readily frozen or archiv...

Biology
BIOL484
Bruce Reed
BIOL 484 Midterm: BIOL 484 midterm notes 1

Does haploinsufficiency related to dominance as there is only one copy of the gene so by definition its phenotype is evident since there is only one copy? Why is it that moving a gene to a new location is considered to be ...

Biology
BIOL484
Bruce Reed

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