KIN 205 Study Guide - Final Guide: Oil Red O, Calcium Atpase, Alpha-Amylase
13 Dec 2017
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Types of histochemical staining: fiber type, myofibrillar atpase (myosin atpase actomyosin, myosin) Summary table of fiber type and metabolic properties. Light medium: subdivided into iia, iix (d) Iia > oxidative capacity of iix or b. Iib have greatest glycolytic capacity * not found in humans. Human muscle biopsy and initial preparation of tissue. Local anaesthetic injected into skin, 1cm or smaller incision made. Centre plunger is then raised- opens window for muscle to migrate into hollow needle. Plunger is lowered cuts off piece of muscle. If used for biochemical analysis (metabolite concentrations or enzymatic analysis) then its frozen immediately. If used for histochemical analysis retracted muscle is removed and mounted accordingly: This maintains the fiber arrangement prior to freezing and provides hard surface before slicing. Skeletal muscle has been classified by contractile sing myofibrillar aptase activity. Cross sections are incubated with atp and calcium, 9. 4 ph.
