KIN217 Study Guide - Low-Density Lipoprotein, Serum Albumin, Apolipoprotein A1

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Published on 31 Jul 2012
School
University of Waterloo
Department
Kinesiology
Course
KIN217
Professor
Kin 217 – October 26 th
Go HANNAH
Paradox of Names
-Why do Low Density Lipoproteins have higher density of TAG and Cholesterol than High
density lipoproteins?
- What happens when you centrifuge LDL and HDL
-Apolipoproteins
Apo-A
-approximately 50-60% OF hdl MW
Apo-B
-20-25% of LDL mw
Electrophoresis
- decrease in diameter…HDL moves faster than LDL
- The major apoproteins in HDL are Apo A, C, E
- The only apoprotein in LDL is Apo-B00
- The mw of Apo-B is greater than Apo-A I or II
- BUT Apo-B takes up only about 20% of LDL, while Apo-A takes up about
50% of HDL
- The def of HDL and LD arise from the centrifugation results floating = LDL
(between 1.996 to 1.063 g/ml) and sedimenting
-Release of free fatty acids?
oHormone-sensitive lipase
Adipose cells have
Activated by glucagons
Inhibited by insulin
oFree fatty acids in blood
Normally low levels
Serum albumin will bind most
Small pool exists
-Diagnostic use of lipid and lipoprotein values
oMost doctors don’t measure LDL cholesterol
oTake total cholesterol measurement and use spectrometer..
They’ll measure HDL cholesterol
And then measure triacylglycerol (the backbone – glycerol)
oFriedwald formula for the calculation of LDL concentration in plasma
LDL (mg/dL) – US – HDL – Triglyceride/5
LDL (mmol/L) – What we use (much smaller values) - HDL –
Triglyceride/2.2
oNeed to think about whether a test is feasible (if too expensive, not feasible)
-Structure of glycogen
oSeries of figures from text
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Document Summary

What happens when you centrifuge ldl and hdl. Electrophoresis decrease in diameter hdl moves faster than ldl. The major apoproteins in hdl are apo a, c, e. The only apoprotein in ldl is apo-b00. The mw of apo-b is greater than apo-a i or ii. But apo-b takes up only about 20% of ldl, while apo-a takes up about. The def of hdl and ld arise from the centrifugation results floating = ldl (between 1. 996 to 1. 063 g/ml) and sedimenting. Release of free fatty acids: hormone-sensitive lipase. Inhibited by insulin: free fatty acids in blood. Serum albumin will bind most: small pool exists. Diagnostic use of lipid and lipoprotein values: most doctors don"t measure ldl cholesterol, take total cholesterol measurement and use spectrometer And then measure triacylglycerol (the backbone glycerol: friedwald formula for the calculation of ldl concentration in plasma. Ldl (mg/dl) us hdl triglyceride/5. Ldl (mmol/l) what we use (much smaller values) - hdl .

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