KIN217 Study Guide - Low-Density Lipoprotein, Serum Albumin, Apolipoprotein A1
Document Summary
What happens when you centrifuge ldl and hdl. Electrophoresis decrease in diameter hdl moves faster than ldl. The major apoproteins in hdl are apo a, c, e. The only apoprotein in ldl is apo-b00. The mw of apo-b is greater than apo-a i or ii. But apo-b takes up only about 20% of ldl, while apo-a takes up about. The def of hdl and ld arise from the centrifugation results floating = ldl (between 1. 996 to 1. 063 g/ml) and sedimenting. Release of free fatty acids: hormone-sensitive lipase. Inhibited by insulin: free fatty acids in blood. Serum albumin will bind most: small pool exists. Diagnostic use of lipid and lipoprotein values: most doctors don"t measure ldl cholesterol, take total cholesterol measurement and use spectrometer And then measure triacylglycerol (the backbone glycerol: friedwald formula for the calculation of ldl concentration in plasma. Ldl (mg/dl) us hdl triglyceride/5. Ldl (mmol/l) what we use (much smaller values) - hdl .
