BIOL 112 Study Guide - Midterm Guide: Methionine, Transfer Rna, Guanine

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BIOL 112 Full Course Notes

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Learning goals week 5: understand the central dogma and describe the step of information flow. The central dogma of biology states that information flows from the dna (information storage) to the mrna (information carries) through a process called transcription. The information is then translated through to proteins in a process called translated. In essence, dna is transcripted to create mrna, which is translated to form proteins (active cell machinery): understand the differences in chromosome, gene expression and arrangements between eukaryotic and prokaryotic cells. In prokaryotic cells, gene expression is a process that happened as the proteins are being made. Transcription and translation are happening at the same time. Transcription and translation happen in a different place and time in eukaryotic cells. Genetic material is supercoiled form in prokaryotic cells and is in the form or chromosomes condensed to chromatin in eukaryotic cells: describe the general features of gene organization on eukaryotic and prokaryotic chromosomes.

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1 DNA sequences found in introns provide [A] amino acid sequence information.

[B] regulatory information.
[C] no known useful information.

[D] structure for the gene.
[E] alternative DNA splicing possibilities.

3. The TATA box is a(n)
[A] sequence close to the promoter region of many genes.
[B] square-shaped sequence.
[C] enhancer consensus sequence.
[D] activator sequence necessary for proper translation.
[E] None of the above

5. Regarding genes and gene expression, which of the following is not a difference between prokaryotes and eukaryotes
[A] Eukaryotic DNA does not contain a TATA box close to the initiation site as does prokaryotic DNA.

[B] In eukaryotes, RNA polymerase requires

'transcription factors'or initiation.

[C] Prokaryotic genes are often grouped together in

operons.

[D] In eukaryotes, the regulation of several genes at once requires common control elements in each of the genes.
[E] There is a diversity of eukaryotic polymerases that is reflected in the diversity of eukaryotic promoters.

10) In eukaryotic cells, a repressor

[A] is made of DNA.
[B] binds to the enhancer region to block transcription. [

C] is located both upstream and downstream from the promoter.
[D] binds to the operator to block RNA polymerase.

[E] binds to a silencer to reduce transcription rates.

Coordinated regulation of genes in eukaryotic cells [A] is the result of positioning the same regulatory sequence in front of each gene.
[B] results from all of those genes being under the control of one promoter.

[C] occurs because related genes all have the same

operons.
[D] occurs because enhancers cause DNA to bend.

1. A region of a chromosome contains two strands of DNA, yet only one is used to transcribe a gene because

A. all genes are found on the same strand of DNA in a chromosome.

B.both strands are transcribed, but only one strand of DNA contains a reading frame.

C.RNA polymerase binds to the terminator, stopping transcription on one strand of DNA.

D.RNA polymerase binds to the promoter, which precedes the gene only on the template strand of DNA.

E.the start of transcription is random along the chromosome and doesn't always occur on a gene.

2. In eukaryotes an mRNA molecule can only be translated by one ribosome at a time.

True or False

3.The process by which cells use the information of RNA molecules to make proteins is transcription.

True or False

QUESTION 3

What is the purpose of chromatin-remodeling enzymes in human cells?

The enzymes control the rate of translation of an mRNA transcript

The enzymes add phosphate or methyl groups to histone tails

The enzymes slide nucleosomes farther apart or closer to each other

The enzymes send damaged proteins to the proteasome for degradation

The enzymes cause DNA looping to occur

QUESTION 10

If you can drink milk as an adult, it means that you have inherited a mutation in the an enhancer for your lactase gene (the gene that encodes the enzyme you need to break down lactose). Predict the effect of this mutation:

The mutation changes the number of domains in the enzyme, which makes it work more efficiently

The mutation changes the amino acid sequence of the lactase protein

The mutation increases the number of copies of the lactase gene that will be found in your genome

The mutation changes whether the lactase sequence is found in an intron or exon

The mutation affects the expression of the lactase gene

QUESTION 12

What determines where in the genome a transcription regulator will bind?

Transcription regulators bind to the 5' UTR region of a gene

Regulators bind via complementary base-pairing to certain DNA molecules

Covalent bonds form between the transcription regulator and the atoms of the DNA backbone

Every eukaryotic gene has a different transcription regulator that will bind to the 5' end of the gene

Transcription regulators bind to specific DNA sequences via multiple weak non-covalent interactions

QUESTION 15

What is the histone code used for?

		Phosphorylation and acetylation of DNA affect its ability to be compacted
		Changes to the sequence of DNA change whether DNA will wrap around histone proteins
		Covalent modifications of histones affect the ability of the transcription initiation complex to form
		Histones provide the codon sequences needed for translation to occur
		The histone code affects which amino acids will get added to a polypeptide

BIOL 112 Study Guide - Midterm Guide: Methionine, Transfer Rna, Guanine

BIOL 112 Full Course Notes

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