WebCT Assignment #2 Feb 27 to Mar 3
Number of questions: 10
PLEASE NOTE: WebCT randomizes order of answers.
Question 1 (1 point)
In a single PCR cycle consisting of 15 seconds at 94°C, 30 seconds at 50°C, and 1 min at 72°C, what is
happening in the step run at 50°C?
a. Primers are being denatured.
b. DNA polymerase is being inactivated.
c. Primers are annealing to the DNA to be amplified.
d. The DNA to be amplified is being denatured.
e. DNA polymerase is extending new DNA from the primers.
Question 2 (1 point)
The restriction enzymes BamHI and BclI cut at the points indicated by arrows. If one sample of DNA was
cut with BamHI and another with BclI, and these two samples were mixed and treated with DNA ligase,
what would occur?
a. No DNAs would be ligated (joined together).
b. Only the BamHI‐cut DNA would be ligated.
c. Only the BclI‐cut DNA would be ligated.
d. Both BamHI‐cut and BclI‐cut DNAs would be ligated, but only to DNA fragments cut with the
same enzyme (e.g., BamHI fragments would be ligated only to other BamHI fragments).
e. Both BamHI‐cut and BclI‐cut DNAs would be ligated with no preference for which fragment is
ligated to which other.
Question 3 (1 point)
Which is not a role proteins play in organisms?
a. store genetic information
b. structural support
c. chemical signaling
d. movement and shape changes
Question 4 (1 point)
Amino acid side chains (R groups) with what characteristic(s) dissolve best in water?
a. the presence of sulfur
b. at least one ring structure
c. polarity or charged structures
d. small sizes and simple structures Question 5 (1 point)
How does the structure of an amino acid enable it to play its most important roles in cells?
a. Because each amino acid contains a variety of functional groups, they can participate in a wide
variety of chemical reactions.
b. It can serve a wide variety of functions in a cell, because it contains the atoms most commonly
found in organisms (C, H, N, and O).
c. Because both carboxyl and amino groups are present, polymerization is exergonic. In addition,
the presence of a side