Practice Final Exam These are questions from Trevor Charles past exams. Variants of these exact questions will most likely remain on his future exams. Use this to prepare for what material to know and HOW to approach his questions, as they can be tricky.

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16 Oct 2011

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The following questions were made based on material from past exams. No
answers are provided as this study tool is meant to act as a practice exam.
1. A microbial culture wherein growth medium flows through at a constant rate and the
cells remain in exponential phase is
a.) a chemostate culture
b.) an enrichment culture
c.) a batch culture
d.) a turbidostat culture
e.) none of the above
2. DNA absorbs ultraviolet light maximally at a wavelength of
a.) 260 µm
b.) 260 nm
c.) 280 µm
d.) 280 nm
3.) 600 nm
3. F plasmid transfer is an example of
a.) conjugation
b.) mutation
c.) homologous recombination
d.) transformation
e.) antibiotic resistance
4. The decimal reduction time is the time required…
a.) to kill a microbial population at a given temperature
b.) to decrease the microbial population by 10% at a given temperature
c.) to decrease the microbial population by 90% at a given temperature
d.) to decrease the microbial population by 1 log at a given temperature
e.) c and d
5. The lowest concentration of an antimicrobial agent that is effective in preventing
growth of a given bacterium is…
a.) the D10 value
b.) the minimal bactericidal concentration
c.) the acceptable use dilution
d.) the minimum inhibitory concentration
e.) the phenol coefficient
6. Autoclaving
a.) is effective in inactivating spores because spores are sensitive to high pressures
b.) is a process commonly used in pasteurization of milk
c.) achieves a pressure of 121 pounds/square inch
d.) is not as effective for sterilization as dry heat
e.) none of the above are true
7. For a given organism, the relationship between time of heating and extent of killing
a.) dependent on the initial cell concentration
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b.) dependent on the pressure
c.) exponential on the pressure
d.) a and c
e.) b and c
8. Pasteurization…
a.) kills all organisms
b.) kills all pathogenic organisms
c.) prevents the growth of all pathogenic organisms
d.) reduces the total number of microbial cells
e.) involves heating at 71ºC for 15 minutes
9. Sterilization using ionizing radiation…
a.) results in the removal of contaminating organisms
b.) results in the object becoming radioactive
c.) is not useful for heat sensitive objects
d.) only affects the DNA of the cell
e.) none of the above
10. Filtration…
a.) can be used to sterilize solids
b.) is especially effective at removing viruses
c.) removes cells based on size
d.) is not particularly effective at sterilizing growth media
e.) c and d
11. The science of Taxonomy is concerned with…
a.) the relationships between organisms
b.) the evolution of organisms
c.) the classification of organisms
d.) the origin of life
e.) the GST and PST
12. Phenotypic analysis
a.) always results in phylogenetically valid groupings
b.) has shown that DNA is often transferred between organisms that are distantly related
c.) has provided the tools that allow the probing of community structure without culturing
the organisms
d.) stipulates that if two organisms have the same %GC, they are the same species.
e.) none of the above
13. Comparison of small subunit rRNA sequences has revealed that, phylogenetically.
a.) yeast are more closely related to methanotrophs than they are to wheat.
b.) cabbage are more closely related to cats then they are to hyperthermophiles
c.) the diversity of prokaryotic organism is much less than the diversity of eukaryotic
d.) none of the above
e.) b and c
14. The size of the most useful RNA molecule for prokaryotic evolutionary studies is…
a.) 5S
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b.) 160S
c.) 16S
d.) 18S
e.) 23S
15. Identification of the three lines of descent, Bacteria, Archaea, and Eukarya, has
come about primarily as a result of…
a.) Advanced methods of morphological examination.
b.) Biochemical methods
c.) Analysis of metabolic pathways
d.) Nucleic acid sequencing studies
e.) Genome sequencing projects.
16. The Proteobacteria…
a.) are gram positive organisms
b.) are eukaryotic organisms
c.) do not include any photosynthetic organism
d.) are all purple in colour
e.) are gram negative organisms
17. Purple phototrophic bacteria
a.) can carry out oxygenic photosynthesis
b.) can photosynthesize in the absence of O2
c.) cannot grow aerobically
d.) were responsible for creation of the Earth’s oxygen atmosphere
e.) b and d
18. Purple nonsulfur bacteria
a.) are photoautotrophs
b.) cannot fix N2
c.) can reduce CO2
d.) store S0 in the periplasm
e.) none of the above
19. Methanotrophs
a.) are obligate anaerobes
b.) are responsible for production of methane in cows
c.) cannot utilize compounds which contain carbon-carbon bonds
d.) none of the above
e.) a and c
20. Pseudomonads
a.) are able to break down many different types of polymeric molecules
b.) are all very closely related, in phylogenetic terms
c.) include some human pathogens, but no plant pathogens
d.) are obligate anaerobes
e.) none of the above
21. Members of the Azotobacter genus
a.) are able to fix N2 aerobically because they contain an O2-insensitive nitrogenise
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