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Final Exam Review Questions

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BIOL 200
Ninan Abraham

Question 1. Circle the correct answer on the exam. A. Proteins meant to remain resident within the rough endoplasmic reticulum are sometimes transported to the Golgi. They may be retrieved from the Golgi by: (2 marks) a. COP1 - coated vesicles b. COP2 - coated vesicles c. Clathrin + adaptin - coated vesicles d. Medial vesicles e. Receptor mediated endocytosis B. If a kinetochore on one side of a metaphase chromosome is destroyed during metaphase, the chromosome would: (2 marks) a. Stop in place b . Stop in the center of the cell c. Attach to another microtubule d. Move to the opposite pole e. Decondense C. As they emerge from the ribosome, signal sequences are recognized and bound by: (2 marks) a. Signal peptidase b. Signal recognition particle c. tRNA d. Signal recognition particle receptor e. Small nuclear ribonucleo proteins (snRNPs) D. A mutant strain of yeast possesses a mutation in the nda2 gene, resulting in no production of alpha - tubulin. As a result, these cells would most likely: (2 marks) a. Not duplicate their chromosomes b. Not enter G1 phase c. Not enter S phase d. Keep the nuclear membrane intact e. Have little or no spindle formation Question 2. (4 marks) Name one mechanism by which each of the following items could be imported into an animal cell. a) A bacterial cell: _______________________________________________________ b) Glucose: _______________________________________________ ______________ c) Cholesterol: _________________________________________________________ d) Na+ ions: ___________________________________________________________ Answer questions 4 to 12 on the examination booklet. Question 4. (4 marks) Answ er in the examination booklet. Tunicamycin is an inhibitor of glycosylation. What would be the fate of a lysosomal protein if cells were treated with tunicamycin. Explain your answer. Question 5. (7 marks) Answer on the examination booklet. ATP synthase co nsists of two multisubunit complexes, F0 and F1. Identify where each complex is located in both mitochondria and chloroplasts and describe the function of each complex. Be specific. You may use a labeled drawing to answer this question. 6. (10 marks). Answer part a, b and c on this page and part d on the examination booklet. You have discovered a new plasma membrane protein, B200, which is found on the surface of B cells. Given your knowledge of the protein translocation process, annotate the dia grams (domain maps) appropriately indicating the position of any membrane crossing domains sequence, (signal start transfer sequences and stop transfer sequences): a. If B200 was a transmembrane protein with a single membrane spanning domain with the N -term
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