BIOL 200 Study Guide - Midterm Guide: Green Fluorescent Protein, Lipid Bilayer, Electron Microscope
Document Summary
Discrete bands, repeating structure, shorter dna fragments (sds page/nuclease digestion) Control, histones are degraded not digested, dna is digested by nuclease. Resolution: defined as the closest spacing of two points which can be distinguished as separate entities. Note: the smaller you get, the more magnification and resolution you need to see an object. Easy to tag protein of interest and can be tracked over time (live sample: tem, sem. Ca(cid:374)"t see s(cid:373)all thi(cid:374)gs e. g. organelles, ribosomes, etc. Details of cytoplasm (samples cut into thin sections) Difficult to know 3d shape of structures. Resolution limited wavelength of light (0. 2 um) Black & white (may have superimposed color) Resolution limited by electron beam (0. 2 nm) Can be used to see very small things. Why do we need biological membranes: boundary and permeability barrier, organization and localization of function (i. e. organelle compartmentalization, transport processes (protein channels, signal detection (receptors, cell-to-cell communication. The membrane has different permeability for different types of molecules.
