BIOL 362 Study Guide - Protein Filament, Atp Hydrolysis, Tubulin
Document Summary
Cyoskeleton-network of cytoplasmic protein filaments of eukaryotic cell that gives cell shape and the capacity for movement. Stathmin: binds to tubulin units and sequesters them prevent polymerization. Microtubule accessory proteins: stabilizers, xmap215: longer less dynamic mt. +tips aid in binding of mt to cell cortex. Actin: 10 nm diameter, f-actin/g-actin monomer, muscle contraction, motility, transport. Arp2/3: branched arrays at 70% binding to minus end o o network (lamellapodia) Actin arp"s: thymosin inhibits polymerization by competing with profiling, profilin helps formin find new monomers adding them to plus end. Actin accessory proteins: destabilizers, gelsolin: sever filament and bind to plus end (dual fx). Intermediate filaments: 10nm diameter, variable monomer, structural support, shape, strength. Kinesin (mt)-use atp hydrolysis to detach from mt: plus end directed, kin 1/3= organellar movement, kin 5=sliding mt in mitosis, kin13= mt destabilizer, kin14=minus end directed cargo transport.
