CHBE 251 Quiz: CHBE 251 tut 5 Jan 30 2019

Genetics question: determine the wild type mRNA?

A fragment of a wild-type polypeptide is sequenced for sevenamino acids. The same polypeptide region is sequenced in fourmutants.
Wild-type N . . . Thr-His-Ser-Gly-Leu-Lys-Ala . . . Cpolypeptide
Mutant 1 N . . . Thr-His-Ser-Val-Leu-Lys-Ala . . . C
Mutant 2 N. . . Thr-His-Ser-C
Mutant 3 N . . . Thr-Thr-Leu-Asp-C
Mutant 4 N . . . Thr-Gln-Leu-Trp-Ile-Glu-Gly . . . C

Determine the wild-type mRNA sequence.

I have already tried:
5'-A-C-U-C-A-U-A-G-U-G-G-U-C-U-U-A-A-A-G-C-U-3'

5

A solution of 5mg/ml is diluted to 1:20 and 5 microliters in a200 microliter reaction is used to generate 3 U. Find U/ml of theundiluted enzyme and what is the specific activity of theenzyme?