FNH 302 Study Guide - Final Guide: Light-Year

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11 Dec 2014
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Sample preparationsize reduction (portion represent sample&population), pretreatment)3elution:biomolecules released from hydrophobic surface by change ( 3),universal b/c most molecules have diff. thermal conductivity relative to helium. Grinding: sample size&achieve buffer composition increase the hydrophobicity sample are desorbed by carrier; Fid=flame ionization detectior,destructive,linear range= 11 ( homogenization,preservation(moist,light, a gradient to #of hydrophobic groups on their surface 4regeneration: removes all. 4),specific:combustible:h c species,measure response based on # of carbon that heat,air(o2),container,preservative,refriged&freeze enzymatic bound molecules ensure full capacity of s next reduce combustion;combinetcd fid:general research; Electron capture inactivation, limit lipid oxidation,microbial growth and contamination) Dynamic exchange ions(na+/cl )2: buffer same ph&ionic strength as starting buffer 3: ionic strength w/ nacl to desorb bound proteins proteins desorbed relative. :pore size of the pckg &surface density of support determine with m) qualitative:retention time t0(blank)=adjusted~quantitative: peak resolution (areas, height h) w 1/2. =width of the band at 1/2h, Size exclusion(sec) (polymers+pro)s:material of controlled pore size:additional the limiting resolution and loading capacity. travel length for small later.

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