MICB 325 Study Guide - Quiz Guide: Mhc Class I Polypeptide-Related Sequence B, Antimicrobial Resistance, Start Codon
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1: directed mutagenesis (knockouts) of specific genes in genomes, as of march 2013, 2604 prokaryotic genomes have been completely sequenced, about 16334 are partially done see http://www. ncbi. nlm. nih. gov/genome/browse/ Result = lots of genes and lots of orfs (thousands, even in a single, bacterial genome). On average, ~50% of the orfs in a newly-sequenced bacterial genome encode proteins of unknown function. As you can see below, it appears that crti is the first gene in an operon: In the above figure the vertical lines represent stop codons in each reading frame; image was obtained using artemis software (freely available at http://www. sanger. ac. uk/resources/software/artemis/) and the r. capsulatus genbank file (available at http://www. ncbi. nlm. nih. gov/genome/1096?project_id=47509). Overlap of crti stop and ctb start codons indicates that they are in the same operon. The two central lines, with orfs represented as white boxes, represent the two directions of transcription. The numbers in between indicate the distance in bp from the predicted origin of replication.
