MICB 325 Study Guide - Quiz Guide: Protein Tag, Mhc Class I Polypeptide-Related Sequence B, Start Codon
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1: as an upcoming graduate of ubc, you should have at least heard something about michael smith"s invention of the method called site-directed mutagenesis. Michael was a prof in the dept. of biochemistry here, and was awarded the. Nobel prize (1993) for his work the only nobel prize ever obtained by someone while they were still at ubc, and thus the only nobel prize given to ubc": http://nobelprize. org/nobel_prizes/chemistry/laureates/1993/smith-autobio. html. The easiest way to explain site-directed mutagenesis is to make the point that oligonucleotides (as in pcr) may bind to a dna sequence even if the base-pairing is not perfect. What is needed is about 10-20 perfect matches on either side of a mismatch to allow an oligo to bind specifically to a target sequence: There are commercially available kits for this purpose: Oligo h-bonded to one strand of cloned gene, in vitro. One strand of coding sequence of cloned gene.
