T HE U NIVERSITY O F
B RITISH C OLUMBIA
M ICROBIOLOGY 202 PRACTICE
E XAM -STYLE Q UESTIONS – IROLOGY
Recommended time for completion: 1 hour, 15 minutes
NAME :________________________ STUDENT #__________________
FAILURE TO FOLLOW INSTRUCTIONS WILL DELAY THE GRADING OF YOUR EXAM
1. All students must adhere to the Faculty of Science rules governing formal examinations (see next page).
2. This exam has 33 questions.-choice questions have one answer. Other multiple-
choice questions will require you to select two or more answers – this is indicated in the question stem
(all must be correct in order to receive the point). This exam has 5 double-sided pages including the
3. Record all multiple choice responses IN DARK PENCIL on the computer answer sheet — NOT on
the question paper. Rough work may be done on the question paper, but nothing written on it will be
4. Make sure to fill in the bubbles for your name (Family name FIRST, Given name LAST) and your 8
digit student number IN PENCIL. Other student information is NOT required. IN INK, sign the
question paper and the answer sheet in the spaNOT be marked if thisam paper will
is not done correctly.
5. Students MUST record their answers onto their answer shNOt during the time allowed for the exam.
writing will be permitted after the time allowed for the exam has expired.NO appeals will be considered.
6. The question paperMUST be returned withthe answer sheet. An answer sheet without question paper
will NOT be graded. In this regard, students areSTRONGLY CAUTIONED to guard against theft of
their question paper by other students. NO appeal relating to loss of a question paper by theft will be
7. The invigilators willNOT answer any clarifStudents must interprethe exam.
and answer each question on their own.
8.NOTE: There will be questions that require one answer bles to fillrs. You will be told how many bub
in for each question. Actual exam questions will have a maximum of 5 answers to choose from and you will not see
the phrase “For this question, pick as many answers as necessary to correctly answer the question”. 2
Faculty of Science Rules Governing Formal Examinations
#1. Each candidate must be prepared to produce, upon request, a Library/AMS card for identification.
#2. Candidates are not permitted to ask questions of the invigilators, except in cases of supposed errors or
ambiguities in examination questions.
#3. No candidate shall be permitted to enter the examination room after the expiration of on -half hour from
the scheduled starting time, or to leave during the first half hour of the examination.
#4. Candidates suspected of any of the following, or similar, dishonest practices shall be immediately
dismissed from the examination and shall be liable to disciplinary action:
• Having at the place of writing any books, papers or memoranda, calculators, computers, audioro
video cassette players or other memory aid devices, other than those authorized by the examiners.
• Speaking or communicating with other candidates.
• Purposely exposing written papers to the view of other candidates. The plea of accident or
forgetfulness shall not be received.
#5. Candidates must not destroy or mutilate any examination material; must hand in all examination papers;
and must not take any examination material from the examination room without permission of the
READ EACH QUESTION STEM CAREFULLY. THERE ARE NO PARTIAL MARKS
FOR QUESTIONS REQUIRING MORE THAN ONE ANSWER. You will not see the phrase
“For this question, pick as many answers as necessary to correctly answer the question” in the
actual exam, but they are a very good way to test your knowledge of the material which is why
they are used here. Actual exam questions will have a maximum of 5 answers to choose from.
1. Most scientists do not consider viruses to be "alive" because:
(a) They have no genes.
(b) Their metabolic machinery is borrowed from the host cell.
(c) They are unable to reproduce.
(d) No definite structural features are seen under the microscope.
(e) They are unable to evolve.
2. Which of the following statements about viral capsids is FALSE?
(a) Damage to one of the protein subunits of a virus capsid may destroy the infectivity of the
(b) One function of the outer shell (capsid) of a virus particle is to protect the virus genome
from chemical damage.
(c) The outer surface of the virus is responsible for recognition of the host cell.
(d) One function of the outer shell (capsid) of a virus particle is to protect the virus genome
from enzymatic damage.
(e) One function of the outer shell (capsid) of a virus particle is to protect the virus genome
from physical damage.
3. For this question, pick as many answers as necessary to correctly answer the question.
Which of the following statement(s) would correctly finish the statement. "Viruses
(a) are carriers of genetic information.
(b) are obligate intracellular parasites.
(c) all have lipid bilayer envelopes.
(d) can replicate on their own.
(e) can reproduce within a host cell.
(f) utilize double-stranded DNA genomes at some point in their replication cycle.
4. For this question, pick as many answers as necessary to correctly answer the question.
A "permissive" viral infection always results in which of the following outcomes?
(a) Inhibition of cellular protein synthesis.
(b) Transformation of the host cell into a tumour cell.
(c) Inhibition of cellular DNA replication.
(d) Integration of the viral genome into the host cell genome.
(e) Death of the host cell.
(f) Production of progeny virus. 4
5. Which two of the following statements BEST describes all types of viruses that have an RNA
(a) They always contain a gene encoding an RNA-dependent RNA or DNA polymerase.
(b) The viral genomes can always act as mRNA.
(c) The virions of RNA viruses always contain an RNA-dependent RNA or DNA
(d) They are always single stranded.
(e) They have higher mutation frequencies relative to viruses containing DNA genomes.
6. Most enveloped viruses obtain their envelopes by:
(a) "stealing" and then modifying vesicles that may be used in the exporting of cellular
(b) breaking down the membrane of the cell they infect and incorporating the lipid into the
(c) budding through the cell’s membrane as they leave an infected cell.
(d) encoding an enzyme which synthesize the necessary lipids for the envelope.
(e) finding pieces of cell membrane released from dead cells and wrapping it around them.
7. Which of the following most accurately describes a latent infection caused by a virus?
(a) The virus replicates in the host and the host cell is usually killed by the release of the
(b) The virus genome has integrated into the host cell, and possibly transformed the cells into
(c) The viral genome is inside the cell, but the genome is not replicating or the virus is not
doing harm to the cell.
(d) The virus replicates in the host cell, and slowly releases virus progeny with very few of
the infected cells dying.
(e) The viruses infects the host cells, produces a virus progeny, then completely stops and
8. Which one of the following hypothetical viruses cannot replicate successfully in a host cell?
Assume the capsid proteins are encoded in all viruses’ genomes, and if the virus can produce
mRNA, it will be translated and the polyprotein will be cleaved properly.
(a) The virus has a single stranded (–) DNA genome and replicates in the cytoplasm. It has a
DNA and RNA polymerases packaged inside the capsid.
(b) The virus has a single stranded (–) DNA genome and replicates in the cytoplasm. It has a
DNA polymerase packaged inside the capsid, and its genome encodes an RNA
(c) The virus has a double stranded DNA genome and replicates in the host nucleus.
(d) The virus has a single stranded (+) RNA genome and replicates in the cytoplasm of the
(e) The virus has a single stranded (–) RNA genome and replicates in the cytoplasm. It has
an RNA dependent RNA polymerase enzyme packaged in its capsid. 5
9. Which two of the following statements with regards to the reproduction of animal viruses is
(a) When a virus attaches to a host cell, the viral genome is “injected” through the cell
membrane into the cytoplasm.
(b) The specificity of viruses for certain types of cells is usually explained by the virus
receptors that it displays on its surface.
(c) Negative strand ssRNA employ host RNA polymerase to synthesize mRNA.
(d) Viruses often “trick” host cells by attaching to surface molecules that are normally taken
in by endocytosis.
(e) The replication of the genome of the virus only occurs in the intracellular state.
10. For this question, pick as many answers as necessary to correctly answer the question.
The ability of an animal virus to infect a cell depends primarily on:
(a) whether or not the host cell has a nucleus.
(b) the enzymatic activity of the host cell.
(c) the type of viral nucleic acid.
(d) presence of receptor sites on the cell membrane.
(e) whether or not the cell is actively dividing.
11. Which of the following statements regarding the adsorption of viruses is TRUE?
(a) The process involves random collisions between the virus particle and the cell and is
(b) The process requires energy from the hydrolysis of ATP.
(c) Naked viruses could bind to the lipid bilayer of the host cells via exposed capsid regions.
(d) There is a specific binding of a structure on the virus and a complementary structure on
the host cell.
(e) It describes the fusion of the cell membrane with the envelope of a virus.
12. Which two of the following statements about viruses are NOT TRUE?
(a) Viruses contain DNA or RNA.
(b) The virus’ nucleic acid is surrounded by a protein coat.
(c) Viruses multiply inside living cells using viral mRNA, viral tRNA and viral ribosomes.
(d) Viruses multiply inside dead cells.
(e) Viruses can evolve to become more virulent.
13. In the Baltimore viral classification scheme, which two of the following criteria to
(a) The kind of nucleic acid (DNA or RNA) in the capsid.
(b) The shape of the virus’ capsid.
(c) The size of the virus particle.
(d) The presence or absence of an envelope.
(e) The steps involved in the formation of viral mRNA after the virus has infected the cell. 6
14. For this question, pick as many answers as necessary to correctly answer the question.
The reservoir for Poliovirus is believed to be:
(e) non-human primates
15. Which one of the following statements about Poliovirus infections in humans is FALSE?
(a) The virus can remain latent for long periods of time.
(b) Large amounts of virus are excreted from the body during infection.
(c) The virus replicates in the gut.
(d) The virus can infect cells of the central nervous system.
(e) The virus enters the body through the mouth.
16. Which of the following statements about Poliovirus replication cycle is CORRECT?
(a) The viral genome is replicated by the cellular RNA polymerase enzymes.
(b) As the virus’ polyprotein is synthesized, it folds and parts of the polyprotein become a
functional protease and cleaves the polyprotein during and after its synthesis.
(c) To compete with the cellular mRNA, the virus produces a protease which cleaves part of
the cap binding complex of the cellular mRNA, and then uses it as a primer to make its
(d) As the virus’ polyprotein is synthesized, it uses the host cell proteases to cleave it into the
(e) The virus shuts off cellular protein synthesis by having the internal ribosome entry site
(IRES) bind to the 5’ methylated caps of the cellular mRNA.