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BCH 2333 Study Guide - Final Guide: A405 Road, Reaction Rate, Absorbance

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chocolatemouse92
22 Jan 2020
School
University of Ottawa
Department
Biochemistry
Course
BCH 2333
Professor
Maria Musgaard

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Document Summary

The purpose of this experiment was to determine the activity of the enzyme acid phosphatase on pnpp in the presence of an unknown inhibitor and determine the type of inhibitor used. The purpose of the first experiment was to determine a concentration of acid phosphatase that would allow the reaction to proceed for 10 mins at a constant rate. Based on observed and measured values, a hanes plot will be constructed, from which km and. The effect of the inhibitor on these parameters of the enzyme will allow us to determine the type of inhibitor used. Table 1: formation of pnp( mol) based on time in an acid phosphatase catalyzed reaction. 6 tubes were prepared, each containing 2. 0 ml of 5. 0m koh. A 7th tube, containing its respective amounts of 1. 0m na-acetate buffer, water, and 0. 5 mg/ml acid phosphatase, was pre- incubated at 37 c for two minutes.

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Related Questions

a) The Km of an enzyme of an enzyme-catalyzed reaction is 7.5 M. What substrate concentration will be required to obtain 65% of Vmax for this enzyme? (same enzyme was used in part a and b) (6 points)

b) Calculate the Ki for a competitive inhibitor whose concentration is 7 x10-6 M. The Km in the presence of inhibitor was found to be 1.2x10-5 M. (6 points)

a) The Km of an enzyme of an enzyme-catalyzed reaction is 7.5 M. What substrate concentration will be required to obtain 75% of Vmax for this enzyme? (same enzyme was used in part a and b) (6 points) b) Calculate the Ki for a competitive inhibitor whose concentration is 7.5 x10-6 M. The Km in the presence of inhibitor was found to be 1.25x10-5 M. (6 points)

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1 / 2 Q1. Prostaglandins are a class of eicosanoids, fatty acid derivatives with a variety of extremely potent actions on vertebrate tissues. They are responsible for producing fever and inflammation and its associated pain. Prostaglandins are derived from the 20-carbon fatty acid arachidonic acid in a reaction catalyzed by the enzyme prostaglandin endoperoxide synthase. This enzyme, a cyclooxygenase, uses oxygen to convert arachidonic acid to PGG2, the immediate precursor of many different prostaglandins. A). The kinetic data given below are for the reaction catalyzed by prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmax and Km of the enzyme. [Arachidonic acid] Rate of formation of PGG2 ) Rate of formation of PGG2 with (10 mg/mL ibuprofen) (mM) (mM min−1) (mM min−1 0.5 23.5 16.67 1.0 32.2 25.25 1.5 36.9 30.49 2.5 41.8 37.04 3.5 44.0 38.91 B) Ibuprofen is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting the synthesis of prostaglandins, ibuprofen reduces inflammation and pain. Using the data in the first and third columns of the table, determine the type of inhibition that ibuprofen exerts on prostaglandin endoperoxide synthase. a) What is the Vmax in the absence and presence of inhibitor? b) What is the Km in the absence and presence of inhibitor? c) What kind of inhibitor is it likely to be?