BCH 4122 Study Guide - Hek 293 Cells, Karyopherin, Northern Blot
16 Jun 2012
School
Department
Course
Professor
Document Summary
Suggest other approaches or next step experiments: you should understand what each technique is used for and extrapolate this into new situations. Paper #1: regulation of skeletal myogenesis by association of the mef2 transcription. Mef1 is an e box sequence that binds myod. Calmoudulin is a ubiquitous ca2+ binding protein. Activates the serine/threonine kinase activity of camk. Mef2 and mrfs act cooperatively to activate muscle-specific genes. Note adding myod to cell = myogenesis. Myogenic bhlh factors = activate muscle transcription as heterodimers with ubiquitous bhlh proteins known as e proteins and bind e boxes in regulatory regions of muscle target genes. These associate with mef2 which binds a conserved a/t rich sequence in muscle gene regulatory regions. Binds to hdac by its mad/mef domain. Binding of hdac causes repression of mef2c activity. The repression can be relieved by camk (calcium/calmodulin-dependent protein kinase) Note hdacs class iia (4 and 5) are expressed and enriched in heart, skeletal muscle, and brain.
