BIO 1140 Study Guide - Final Guide: Amoebozoa, Spermatocyte, Interphase
2 May 2015
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Revolving nosepiece: supports objective lenses- simple change in magnification. Stage: supports specimen; knobs to move stage in x & y axis. Coarse focus knob: rapid change in distance b/w specimen & objective, rough focusing- not for 40x + Fine focus knob: small change in distance b/w specimen & objective, allows for final focusing adjustments. Ocular / eye piece: magnifying element of microscope (10x) Look through the ocular to view specimen: parfocal: when switching from one objective to the next, image is not lost. Objectives (4x, 10x, 40x, 100x: magnifying element closest to the specimen, numerical aperture: determines resolving power of the objective. Condenser: system of lenses that concentrates the light created from the laminator, does not magnify the object. Condenser height adjustment knob: allows one to focus the concentrated light onto specimen. Aperture iris diaphragm: reduces glare from unwanted light by adjusting the angle of the cone of light from condenser.
