BIO 2133 Study Guide - Comprehensive Final Exam Guide - Gene, Chromosome, Phenotype

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Polytene chromosome from the isolated salivary gland of the 3rd instar larvae of the fruit fly, drosophila virilis. This is at the magnification of 400x and at room temperature of 25 c. Polytene chromosome after a brief heat shock treatment of 37 c from the isolated salivary gland of the 3rd instar larvae of the fruit fly, drosophila virilis, at a magnification of 400x. Yes, there are visible differences that are observed. These differences are shown through areas of puffing . There should be more puffs in the treatment at 37 c compared to the one at 25 c because with higher temperatures, the levels of gene activity or expression will also increase. Yes, they should be physically interconnected by the chromocenter, where the chromosomes are bundled together, so it looks somewhat like a big knot, but in reality, it"s actually the polytene chromosomes.

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