BIO 3170 Study Guide - Midterm Guide: Nucleic Acid Sequence, Pyrophosphate, Enzyme

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Describe the two steps that are necessary for the rnap to take off from the promoter and to transcribe: release of sigma factor from the holo enzyme (rnap) Allows the af nity for the promoter sequence to decrease, allowing it to leave the promoter: movement of rnap along dna. The structure causes rnap to pause and dissociate from the matrix strand: rho dependent termination: rho binds at the 5" end of the rna to terminate transcription. Activator is controlled by a ligand which can either increase or decrease activator af nity and function: negative: genes are expressed unless a repressor protein is bound to block transcription. Ligand can either reduce or increase repressor af nity for the promoter: de ne the term operon and illustrate key components, operon: group of genes located in close proximity that are regulated by the same promoter, key components:

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