Study Guides (248,350)
Canada (121,501)
Biology (1,271)
BIO3153 (18)
Final

Lecture 10 - EXAM VERSION.docx

4 Pages
106 Views
Unlock Document

Department
Biology
Course
BIO3153
Professor
Michael Jonz
Semester
Fall

Description
Lecture 10EXAM VERSIONNovember22121139 PM Electron MicroscopyTransmission electron microscope TEMScanning electron microscope SEMLight source bombardment of electronsLens electron gun and magnetEffect high resolutionAdvantage resolution 2000X than light microscopeResolves fine structure of cellDisadvantage time consuming prep appropriate technique for some experiments Transmission EMLight source bombardment of electronsLens electron gunUse inside cellsEffect contrast dense imageMechanism Tissue cut and bombarded with electrons some pass through image some bounceCompared to LMSimilar to light but biggerFocuses electrons released from a gun onto specimen instead of using condenser to focus lightProcessElectrons emitted at filament or cathode source of light at top of cylindrical 1columnAccelerated by high voltage in vacuum by air filament by anode1pass through hole to form electron beam that travels down column2Magnetic coils focus electron beam Specimen stained with electron dense material produces electron dense 1imagesElectrons passing through are scattered by stained structuresthe rest are focused to form an imageNote Can increase density with reagentsElectron denseblack electrons scattered away that dont make it to detectorElectron lucent light areas where electrons were allowed to pass through forms image TEM Preptakes days or weeksTissue fixed in glutaraldehyde1Fixative adds densityAddition of OsO4 increases electron density1Dehydration and infiltration with plastic resin 1
More Less

Related notes for BIO3153

Log In


OR

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


OR

By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.


Submit