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BPS 3101 Study Guide - Midterm Guide: Tandem Repeat, Escherichia Coli, Retrotransposon

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peargoldfish669
16 Oct 2011
School
University of Ottawa
Department
Biopharmaceutical sciences
Course
BPS 3101
Professor
Linda Bonen

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Document Summary

Sequence from one clone is used as a probe to screen library of clones to find overlapping ones. Use clone a1 as a probe hybridization on plate of clones a1, f6 and e7. On next grid, use clone f6 as probe hybridization on plate of clones a1, f6 and b12. ___e7__ _____f6___ a1 overlaps with e7 and f6, and f6 but not a1 overlaps with b12, and e7. Note: if there are repetitive sequences on one probe that hybridize to many clones: Instead use short unique sequences mapping close to the end of the clone as a probe. Use dna complementary to repetitive dna to hybridize, so that only unique sequence can hybr. overlaps with a1 but not f6. Design primers for pcr that are based on the sequence at the end of a clone (similar to question 6 on the assignment)

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Related Questions

A microarray consists of a flat matrix with regularly placed Ć¢Ā€ĀœspotsĆ¢Ā€Ā of DNA, each with 107 Ć¢Ā€Ā“ 108 copies of the same DNA sequence. (But each spot has a unique DNA sequence.) You can label fragments of human genomic DNA with a fluorescent dye and hybridized the DNA probes to the microarray. If there are sequences present in the genome that can hybridize to the spot, the spot will fluoresce, which can be detected via microscopy. Indeed, you could use such an array to detect base-pair differences among different individuals, so-called SNPs (single nucleotide polymorphisms). How do you imagine such a system would work? (Hint: think about the how the conditions can affect the hybridization of DNA.)

The base sequences of 2 DNA clones are below. The base sequenceof only 1 strand of each clone is shown. One clone is a cDNA cloneand the other is a genomic DNA clone. Both clones code for the samepolypeptide encoded in the nuclear genome of dogs. Annotate the clones and answer the following questions:

Clone 1:3

a) Can clones in a cDNA library contain 5Ć¢Ā€Ā² UTR sequences? 3Ć¢Ā€Ā² UTR sequences?

b) Would you be likely to find on average longer ORFs in cloned sequences from a genomic library or from a cDNA library? Explain.