BPS 3101 Study Guide - Midterm Guide: Hybridization Probe, Hindiii, Bamhi
7 Mar 2015
School
Department
Course
Professor
Document Summary
You have sequenced the region of an insect genome containing gene x, shown on the restriction map below (black bars = exons, dotted lines = introns). Arrows show the positions of short stretches of that sequence, with potential initiation and termination codons in underlined italics. Restriction sites are e: ecori, b: bamhi, h: hindiii. Fall 2014: show what you would expect to see if exon 3 were used as a hybridization probe in (i) northern analysis and (ii) southern analysis - bamhi (lane 1), ecori (lane 2), hindiii (lane 3) Be sure to indicate size markers on your figures. For extra practice, design primers to generate an rt-pcr product for gene x and give its size(s). If it were used as a probe in (i) a. Use schematics in your answer: explain how you would use race to determine the position of the 3" end of mrna x and show your strategy in schematics.
