BPS 3101 Study Guide - Midterm Guide: Mycobacterium Leprae, Paratuberculosis, Ecori
7 Mar 2015
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Individuals which have a g-to-t mutation within intron 1 (at position of black triangle) Suppose that the human gene x encodes an enzyme which binds drug d through a domain encoded within exon 2. The gene x region is shown below: exons = black, introns = dotted lines; intron sequence = lower case letters; exon sequences = upper case letters with initiation and stop codons in underlined italics. Restriction sites are e: ecori, b: bamhi, h: hindiii. show alternative splicing such that exon 2 is skipped over. Describe two screening methods (one that is dna-based and the other rna-based) to determine whether an individual carries such a mutant allele for gene x. Suppose that the restriction map below shows bacterial gene a (black bar), which encodes a membrane-located transporter needed for drug m to enter the bacterial cell. Arrows show the positions of is elements found in strains #1 to #4.
