BPS3151 Study Guide - Quiz Guide: Sulfur, 5G, Tryptophan
30 Dec 2015
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A 20x dilution with a280 = 0. 7 has an undiluted absorbance of 20x x 0. 7 = 14. Therefore, the protein concentration of the stock solution is approximately 1 x 10-4 m. Sec (u) 0 0. 0% (b) 0 (z) 0 (x) 0. Total number of negatively charged residues (asp + glu): 110. Total number of positively charged residues (arg + lys): 109. Extinction coefficients are in units of m-1 cm-1, at 280 nm measured in water. Abs 0. 1% (=1 g/l) 1. 407, assuming all pairs of cys residues form cystines. Abs 0. 1% (=1 g/l) 1. 399, assuming all cys residues are reduced. The n-terminal of the sequence considered is m (met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). The instability index (ii) is computed to be 40. 43. = (# of tyrosines) * (global extinction coefficient of tyrosine) + (# of tryptophans) * (global extinction coefficient of tryptophan)
