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Extraction and Thin Layer Chromatography.docx

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Department
Chemistry
Course Code
CHM1321
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All

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Extraction and Thin Layer Chromatography
Daniel Amos: 6853559
21/01/13
Introduction
Part A
Extraction of liquid dyes of different solubilities:
In this experiment we will determine the methods of extracting a series of dyes.
Using a separation of an organic phase and an aqueous phase, ether and water
respectively, the goal is to determine the layer that each dye may separate to under given
circumstances.
Separating the dye to a specific layer would allow for extraction of impurities that would
not follow the same path.
Mechanisms: Extraction of water soluble dyes.
- Separating Methylene Blue
- Separating Methyl red
- Separating Methylene Blue and Methyl Red
Mechanisms: The salting out effect
- Salting out Crystal Violet
Part B
Using thin layer chromatography to identify the components of an unknown compound.
In this experiment the composition of an unknown substance is determined using TLC
plates and thin layer chromatography.
The Rf level of individual components in a compound may be determined using thin layer
chromatography by measuring the distance travelled by a sample of the compound.

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Description
Extraction and Thin Layer Chromatography Daniel Amos6853559 210113 IntroductionPart AExtraction of liquid dyes of different solubilitiesIn this experiment we will determine the methods of extracting a series of dyesUsing a separation of an organic phase and an aqueous phase ether and water respectively the goal is to determine the layer that each dye may separate to under given circumstancesSeparating the dye to a specific layer would allow for extraction of impurities that would not follow the same pathMechanisms Extraction of water soluble dyesSeparating Methylene BlueSeparating Methyl redSeparating Methylene Blue and Methyl Red
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