BIO153H5 Study Guide - Midterm Guide: Microscope Slide, Paramecium, Pupillary Distance
Document Summary
Prokaryotic/eukaryotic cells range from 0. 001 mm to 0. 05 mm in diameter, with only the very largest cells (0. 1mm) Microscopes; needed for detecting the existence of most cells and for visualizing cellular components. Two types of microscopes; compound light microscope and dissecting microscope (stereomicroscope) Objective lenses, 3 objective lenses; 4x (scanning lens), 10x (low power), 40x (high power) Total magnification is calculated by multiplying the power of the ocular by the power of the objective. Adjusting interpupillary distance, slide the eyepieces closer together or farther apart until the two fields merge to form a single circle of light. Parfocal means that when you change objective lenses the focus remains sharp and the specimen requires very little refocusing. Parcentric means that when you change objective lenses, the specimen should stay centered in the field of view. Working distance is the distance between the objective lens and the specimen. As the magnification increases your working distance decreases.
