BIO314H5 Final: Lectures 10_11_12
Document Summary
Indicaion of gene funcion - sequence similarity to previously characterized genes. Increased knowledge of infecious organisms, disease development, aging, and other biological processes. Comparaive genomics - genes required for life, diferences among major phyla etc . But how do we get this informaion out of the sequence data: bioinformaics and phylogeneic reconstrucion, funcional genomics. Comparing new gene sequences to previously characterized genes. Genome analysis: controlling sequences, genome structure and evoluion, non-coding rna. Automate detecion and base calling through the use of luorescent ddntp"s to labeled extended strands, electrophoresis in a gel or capillary, detecion by laser excitaion, automated analysis. Major sequencing centers also automate sample prep and other aspects of dna sequencing like reacion set up and reacion puriicaion: huge throughput - one abi3700 ~500,000 bases/day; celera has over 200 3700"s . Similarity searching, including sequence comparison, is one of the principal techniques used by computaional biologists and has found widespread use among biologists in general.
