BIO380H5 Midterm: BIO380 Midterm 1 Notes (Lec 1-10)

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These dyes stain the cells without killing them. The dyes are mixed with agar and put on a slide to dry. Genetic modification can then be traced only to those cells that express it: ex. Green fluorescent protein (gfp), a fluorescently active protein. A gene altered in this way is called a transgene because it contains dna from another species. Parents were infected with a replication-deficient virus that carried an active gene for. The chick inherited and this was expressed in every cell. They transplated the neural tube and neural crest of a gfp-transgenic embryo into a similar region of a normal chick embryo(b). Later, they could see gfp labelled cells migrating into the stomach region(c) and soon the whole gut showed gfp (d) Excises inhibitory dna that represses expression of other colours. Works in a cell by cell specific manner.

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