PSY352H5 Study Guide - Hybridization Probe, Northern Blot, Silent Mutation
Document Summary
Isolate gene from rest of genome: produce large enough. So it is large and difficult to analyze for function: messenger rna. Only coding sequence and axons so much simpler. Converted to dna first before cloning (complementary dna: imp to know sequence and compare to other sequences. Which gene is increased or decreased by influence of a drug to udnerstad adverse effects of drugs and why. For liver toxicity in a rat for example (microaaray shows white line that shoes there is gene expression!) In control we dont see same thing: model organisms are indespinsible tool to study genes, yeast, bacteria, animals, complex more difficult more but more relevant model vecoems to humans, cloning- genetically identical animal. Inbreeding way it is done by lab. Large gain or loss of chromosomal rgions or switching if parts of chromosome (transolocations) Small nucleotide changes (substitutions, deletions or insertions: diff types of single cell mutations.
