Study Guides (390,000)
CA (150,000)
UTSC (10,000)

nucleus, mitochondria


Department
Biological Sciences
Course Code
BIOB10H3
Professor
Rene Harrison

This preview shows half of the first page. to view the full 1 pages of the document.
StructureTo enter: Destinations
Chloroplast1. folded by HSP70 (chaperone
protein)
2. transit peptide binds to its
receptor (located next to a
TOC: translocon of outer
chloroplast membrane)
3. HSP70 in intermembrane
space keeps protein unfolded &
binds to 2nd transit peptide
receptor on inner membrane of
chloroplast
4. move through TIC
(tranlocon of inner membrane)
complex
For Stroma:
- HSP60 refolds protein
and stroma-targeting
domain is cleaved by a
protease
For Thylakoid Lumen:
- stroma-targeting
domain is cleaved to
reveal thylakoid transfer
domain
- protein is transported
into lumen-bacterial
translocon-type
machinery
Mitochondria1. folded by HSP70
2. transit peptide binds to its
receptor (located next to a
TOM: translocon of outer
chloroplast membrane)
For IMM integral
proteins:
- directed to TIM22
complex of IMM
For Matrix:
- directed to TIM23
complex of IMM
- preproten moves
through TIM because of
membrane potential
differences across IMM
- once in matrix ,
preprotein is folded by
mt-HSP70
Nucleus 1. NLS binds to soluble
receptor in cytosol (importin)
2. importin brings protein to
nuclear pore (multi-protein
complex: 30-50 proteins, allows
simple diffusion of small
molecules o/w req NLS)
www.notesolution.com
You're Reading a Preview

Unlock to view full version