BIOB10H3 Study Guide - Midterm Guide: Fluorescence Microscope, Aequorea Victoria, Fluorophore
Document Summary
Methods to look at specific proteins within cells. Fluorescence microscope detects the location of compounds known as fluorophores (fluorescence dye) Fluorophores absorb energy of a specific wavelength (uv light) Releases a portion of this energy as longer visible wavelengths of light called fluorescence. Filter (1) only allow the wavelength that excites the fluorophore to reach the specimen. Filter (2) only allow the longer wavelength light (fluorescence) to reach the eyepiece. Object appears brightly colored against a dark background. A fluorophore could be attached to an antibody that is directed against a specific cellular protein immunofluorescence. Made by vertebrates as a defense against infection human: made by b cells in blood. Each b cells carries a unique type of antibody on its surface that reacts against a specific antigen. When an antibody encounters its antigen, the b cell divides rapidly and secretes lots of antibodies. Fluorophore binds to lysine, amino group etc on the antibodies.
.jpg)
