BIOB10H3 Study Guide - Midterm Guide: Protein Structure, Tethering, Mannose

156 views8 pages

burgundysea-lion52

8 Dec 2017

School

Department

Course

Professor

For unlimited access to Study Guides, a Grade+ subscription is required.

Document Summary

#1: er membrane gef (guanine nucleotide exchange factor) recruits sar i to the er and changed gdp to gtp, gtp causes change in sar i protein structure causing it to bend. Tethering tethered by extended brous proteins and multi protein tethering complexes additional cytosolic proteins that link vesicles to target: rab protein small gtp binding proteins lipid anchor. 60 di erent rabs known associate with speci c membranes. / organelles recruit tethering give certain membranes within cells their identity: docking leads to vesicle fusion (requires snares) #2 v- snares on vesicle membrane t- snares on target membrane interaction between v snares and t snared allows the tethering and docking. Interaction between snares allows for extensive bending that allows the fusion event vesicle membrane becomes continuous with target membrane. Lysosomes can digest macromolecules that make up the ecm in the cell exterior. Lysosome inside acrosome (tip of sperm cell) eats the outside layer of the egg allowing fertilization.

Related Questions

match the following descriptions with the correct term.

A peripheral membrane protein which forms a basket-like structure that is critical in aiding vesicle budding

Clathrin , COP I coat, Translocon, COP II coat

Mediates retrograde trafficking from the Golgi to the ER

Clathrin , COP I coat, Translocon, COP II coat

Mediates vesicle trafficking from the ER to Golgi

Clathrin , COP I coat, Translocon, COP II coat

Serves as a channel through which secretory proteins pass

Clathrin , COP I coat, Translocon, COP II coat

emeraldhound799

The vesicles that transport materials from donor membranes to acceptor membranes include:

	1.	COP-II coated vesicles
	2.	COP-III coated vesicles
	3.	COP-I coated vesicles
	4.	Clathrin coated vesicles

2.5 points

QUESTION 11

During skeletal muscle contraction, the roles of ATP include:

	1.	ATP is not required for forming the crossbridges between myosin and actin filaments.
	2.	ATP is utilized as a structural linker to mediate the interaction between myosin and actin filaments
	3.	Hydrolysis of ATP provides energy for myosin head to move along actin filaments and the binding of ATP to myosin head allows the detachment of myosin fromactin filaments.
	4.	ATP binds to actin filaments to drive the power stroke of myosin head.

2.5 points

QUESTION 12

One of the functions of smooth endoplasmic reticulum is

	1.	Synthesizing steroid hormone in the endocrine cells of the gonad and adrenal cortex
	2.	Serves as the starting point of the biosynthetic pathway
	3.	Synthesizing secretory proteins
	4.	Degrading cell debris

2.5 points

QUESTION 13

The function of COP-II coated vesicles is mainly:

	1.	Transporting materials from endosomes to Golgi complex
	2.	Moving materials from the ER to the ERGIC and Golgi complex.
	3.	Transporting materials from Golgi complex back to endoplasmic reticulum.
	4.	Involved in receptor-mediated endocytosis.

2.5 points

QUESTION 14

Which of the following processes is completed in rough endoplasmic reticulum?

	1.	Chaperones help misfolded proteins fold correctly.
	2.	Proteins that target to nucleus are synthesized in rough endoplasmic reticulum.
	3.	O-linked glycosylation occurs in rough endoplasmic reticulum.
	4.	Misfolded proteins are destructed in rough endoplamic reticulum.

2.5 points

QUESTION 15

Kinesin is the motor protein that coorporates with microtubules. It has the following functions:

	1.	It uses GTP as energy source.
	2.	It can hydrolyze ATP and use the energy to drive cargo along microtubules from minus end to plus end.
	3.	It has a globular head that can act as GTPase
	4.	The tail of kinesin is more diverse and binds to cargo

2.5 points

QUESTION 16

For correct segregation and trafficking, lysosomal proteins are tagged in the cis-Golgi with phosphorylated mannose residues, and then the tagged lysosomal enzymes are recruited to clathrin coated vesicles by mannose 6-phosphate receptors.

True

False

2.5 points

QUESTION 17

The process that specialized cells, such macrophages, engulf relatively large particles is:

	1.	Called pinocytosis
	2.	Called phagocytosis
	3.	Mediated by COP-II coated vesicles
	4.	Called autophagy

2.5 points

QUESTION 18

Proteins synthesized on rough endoplasmic reticulum and smooth endoplasmic reticulum are imported co-translationally to their destinations

True

False

2.5 points

QUESTION 19

Treatment of cells with a drug that promotes microtubule disassembly disperses Golgi complex into separate stacks of membrane compartments, suggesting that:

	1.	Golgi complex is made of microtubules.
	2.	Microtubules are important for the organization of Golgi complex.
	3.	Microtubules are transported into Golgi complex
	4.	Golgi complex is composed of tubulin proteins

2.5 points

QUESTION 20

Which of the following is correct about signal sequence of a secretory protein?

	1.	It is an âaddress codesâ for protein trafficking pathways
	2.	It is usually located at the C-terminus.
	3.	It targets the ribosome and the protein that is being synthesized to endoplasmic reticulum.
	4.	It is removed in Golgi complex.

apricotgrasshopper117

Which compartments are involved in vesicular transport? How are transport vesicles formed and targeted appropriately? What proteins are involved and what are their roles? What kinds of modifications are added to proteins in the ER? What determines whether a protein stays in the ER or moves on? Be sure to understand how vesicles fuse with their target membranes, and the consequences of fusion for the vesicleâs cargo as well as the proteins and lipids that are in the membrane. Know the structure of the Golgi apparatus and its role in intracellular trafficking. Understand the secretory process, endocytosis, exocytosis, function of the endosomes, and function of the lysosomes. Understand why endocytosis happens and how it can be regulated to take up specific molecules.

BIOB10H3 Study Guide - Midterm Guide: Protein Structure, Tethering, Mannose

Document Summary

Get access

Related Documents

BIOB10H3 Lecture Notes - Lecture 7: Secretion, Cell Membrane, Endocytosis

BIOB10H3 Lecture : Important Terms and Definitions

BIOB10H3 Lecture 7: Transport from Endoplasmic Reticulum to Golgi

Related Questions